Informatics Publishing Limited

R. S. Jadhav ¹, P. N. Kendre ², M. H. Kolhe ¹, S. N. Lateef ², S. M. Shelke ³, R. K. Godge ¹

Affiliations
1 Dept. of Pharmaceutics, Pravara Rural college of Pharmacy, Pravaranagar, IN
2 Sanjivani college of pharmaceutical Education and Research, Kopargaon, IN
3 Research Associate Orchid Pharmaceutical Ltd., Chennai, IN

Abstract

A simple, selective, rapid, precise and economical reverse phase highpressure liquid chromatographic method has been developed for the simultaneous estimation of Ofloxacin and Ornidazole from pharmaceutical formulation. The method was carried out on a Kromasil C₁₈ (5μm, 25 cm X 4.6 mm, i.d.) column, with a mobile phase consisting acetonitrile: phosphate buffer (pH 2.4) in the ratio 80: 20% V/V at a flow rate of 1.0 ml/min. Detection was carried out at 294 nm. The retention time of Ofloxacin and Ornidazole were 2.773 and 5.448 min respectively. The developed method was validated in terms of accuracy, precision, linearity, Limit of detection, Limit of quantitation. The proposed method can be used for estimation of these drugs in combined dosage form for routine analysis.

Keywords

Ofloxacin, Ornidazole, RP-HPLC.

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R. K. Godge ¹, M. C. Damle ¹, S. R. Pattan ¹, P. N. Kendre ¹, S. N. Lateef ¹, P. J. Burange ¹

Affiliations
1 Pravara Rural College of Pharmacy, Pravaranagar, Dist. Ahemadnagar 413 736. (MS), IN

Abstract

A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed for the simultaneous estimation of Pseudoephidrine sulphate and Desloratidine from pharmaceutical formulation by using internal standard Nabumetone. The method was carried out on a HiQ sil C18 W (250mm x 4.6 mm) column, with a mobile phase consisting of Acetonitrile: Methanol :Ammonium acetate (45:40:15 v/v) at a flow rate of 1.5 ml/min. Detection was carried out at 265 nm. The retention time of Pseudoephidrine sulphate, Desloratidine, and Nabumetone were 2.067, 3.050 and 3.750min. Respectively. The developed method was validated in terms of accuracy, precision, linearity, Limit of detection, Limit of quantitation. The proposed method can be used for estimation of these drugs in combined dosage form for routine analysis.

Keywords

Pseudoephidrine Sulphate, Desloratidine, Nabumetone, RP-HPLC.

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